Regulatory

Part:BBa_J100110:Design

Designed by: Jessica Gronniger   Group: Campbell M Lab   (2013-06-19)


P5 promoter cloned into scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No internal BsaI sites. The 5' end of Junction B from part BBa_J119301 was changed from TATT to GGCT. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100110.
The 5' end (future Bsa I sticky ends) for Junctions C and D also need to be changed. They will be changed with the next round of iPCR.
The 5' end of Junction C will be changed from TTTC to GTTC.
The 5' end of Junction D will be changed from TTAA to CGGA.

Source

All parts taken from Registry.

References